PENGARUH PEMBERIAN BERBAGAI JENIS ADENDA DALAM MEDIA ½ MS TERHADAP PERTUMBUHAN SEEDLING ANGGREK PHALAENOPSIS IN VITRO

Effects of Different Organic Additives into ½ MS Medium Toward Growth of In Vitro Seedling Phalaenopsis Orchid

Abstract

Growth of in vitro seedling Phalaenopsis orchid can be enhanced by using ½ Murashige and Skoog (MS) medium which was supplemented with organic additive. The purpose of this study is to investigate the effects of organic additives in the ½ MS medium and to determine the best organic of organic additives on Phalaenopsis seedling growth. Culture medium (½ MS) was supplemented with coconut water, banana pulp, carrot extract, tomato extract and pineapple extract. The results showed that ½ MS medium supplemented with coconut water 100 ml/L could increase growth of Phalaenopsis orchid to the number of roots and wet weight. In addition, ½ MS medium supplemented with 200 g/L showed statistically significant to the leaf length. Furthermore, coconut water is the best organic additive for development of in vitro seedling Phalaenopsis orchid.