Antibacterial Activity of Ethyl Acetate Fraction of Methanol Extract Leaves of Orange Leaves (Filicium Decipiens) Against Staphylococcus epidermis

Aktivitas Antibakteri Fraksi Etil Asetat Ekstrak Metanol Daun Kerai Payung (Filicium Decipiens) Terhadap Staphylococcus epidermis

Abstract

The Filicium decipiens (umbrella sunshade) tree, known as ormo kiara ormo or ki soap, belongs to the Sapindaceae family, namely saponin-producing plants which are estimated to have high levels of saponins and toxicity. This study aims to determine the antibacterial activity of the ethyl acetate fraction of parasol leaves against Staphylococcus epidermidis. The extract was macerated using methanol as a solvent. The results of phytochemical screening showed that the parasol leaf contains phenolic compounds, flavonoids, alkaloids, saponins, tannins and steroids. Then proceed with the fractionation process of parasol leaf extract to obtain the n-hexane fraction with liquid:liquid partition using water:ethyl acetate = 1:1 as solvent. The antibacterial activity was tested using the disc diffusion method (Kirby and Bauer diffusion). The concentration variations of the n-hexane fraction were 25%, 50%, 75%, 100%, control (+) cifroploxacin and control (-) DMSO. The highest concentration results were shown at a concentration of 75%, namely 12.52 and a decreasing concentration of 25%, namely 11.12, 100% concentration, 11.02, and 50% concentration, which was 10.62. While the concentration of K(+) namely Cifrofloxacin was 31.95. And shows that the largest concentration is targeted by a concentration of 75% which is 12.52.