ANALISIS EXTRACELLULER PRODUCT Vibrio harveyi BERBASIS NANO-KITOSAN YANG DIPERKAYA EKSTRAK Caulerpa lentillifera SEBAGAI VAKSIN ORAL UNTUK KERAPU CANTANG TERHADAP INFEKSI Vibrio harveyi

Authors

  • Renanda Baghaz Dzulhamdhani Surya Putra, S.Pi., MP., M.Sc PSDKU Akuakultur, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya. Indonesia Author https://orcid.org/0000-0002-3556-0759
  • Dr. Asus Maizar Suryanto Hertika, S.Pi.,MP Program studi Manajemen Sumberdaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya. Indonesi
  • Jedda Ayu Inggrida, SP., MP PSDKU Sosial Ekonomi Pertanian, Fakultas Pertanian, Universitas Brawijaya, Indonesia Author
  • Arendra Nicky Anugerah Putra Mahasiswa PSDKU Akuakultur, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya. Indonesia Author
  • Faris Setiawan Mahasiswa PSDKU Akuakultur, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya. Indonesia Author
  • Alya Madha Su’a Achmadi Mahasiswa PSDKU Akuakultur, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya. Indonesia Author

DOI:

https://doi.org/10.33019/pkq62x73

Keywords:

Vaksin oral, Extraceluller Product (ECP), Caulerpa lentillifera, Nanochitosan

Abstract

Vibriosis merupakan penyakit penting yang menyebabkan tingginya tingkat kematian pada ikan kerapu cantang (Epinephelus fuscoguttatus x Epinephelus lanceolatus), yang sebagian besar disebabkan oleh infeksi Vibrio harveyi. Penelitian ini bertujuan untuk mengevaluasi efektivitas vaksin oral yang mengandung produk ekstraseluler (extracellular product, ECP) dari Vibrio harveyi, nano-kitosan, dan ekstrak Caulerpa lentillifera dalam meningkatkan ketahanan terhadap vibriosis pada ikan kerapu cantang. Rancangan percobaan mencakup pemeliharaan ikan Epinephelus spp. berukuran 10–12 cm selama 30 hari dengan lima perlakuan, yaitu: (A) infeksi Vibrio harveyi (kontrol), (B) ekstrak Caulerpa lentillifera, (C) ECP + nano-kitosan, dan (D) ECP + nano-kitosan + ekstrak Caulerpa lentillifera. Indikator utama yang dievaluasi meliputi tingkat kelangsungan hidup (%), relative percent survival (RPS, %), dan performa pertumbuhan. Hasil penelitian menunjukkan bahwa kombinasi nano-kitosan dan ECP dari Vibrio harveyi yang diperkaya dengan ekstrak Caulerpa lentillifera memberikan respons pertumbuhan yang secara signifikan lebih tinggi dibandingkan perlakuan lainnya. Komposisi tersebut juga secara signifikan meningkatkan tingkat kelangsungan hidup ikan kerapu yang terinfeksi Vibrio harveyi. Temuan ini menunjukkan potensi vaksin oral berbasis nano-kitosan, ECP, dan ekstrak Caulerpa lentillifera sebagai pendekatan pencegahan yang menjanjikan terhadap infeksi Vibrio harveyi dalam akuakultur, sehingga diperlukan penelitian lebih lanjut untuk pengembangan aplikasi komersialnya.

Author Biography

  • Renanda Baghaz Dzulhamdhani Surya Putra, S.Pi., MP., M.Sc, PSDKU Akuakultur, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya. Indonesia

    Renanda Baghaz Dzulhamdhani Surya Putra, S.Pi., M.P., M.Sc is a permanent lecturer at the Aquaculture Study Program, Faculty of Fisheries and Marine Science, Universitas Brawijaya, Kediri Campus, Indonesia. He earned his Bachelor and Master of Fisheries Science degrees from Universitas Brawijaya and obtained a Master of Science in department Biological Science and Technology from National Pingtung University of Science and Technology,  Taiwan, through a competitive international research scholarship.

    His primary research interests focus on aquatic animal immunology, particularly the development of oral vaccines based on recombinant proteins and natural bioactive compounds, probiotic biotechnology, and the application of algal-derived immunostimulants to enhance disease resistance in fish and shrimp. He is also involved in sustainable aquaculture practices, including the utilization of aquaculture waste for environmentally friendly liquid organic fertilizer (POC) production.

    He has published several scientific articles in national accredited journals and international peer-reviewed journals, with topics covering:

    • Recombinant oral vaccines for whiteleg shrimp (Litopenaeus vannamei) against Vibrio harveyi and White Spot Syndrome Virus (WSSV)

    • Immunostimulant potential of Caulerpa lentillifera extracts

    • Nano-chitosan as an effective oral vaccine delivery system

    • Hematological and immune response assessments in fish under environmental stress and pollution

    In addition to his research, he actively contributes to community empowerment projects, scientific seminars, and serves as a reviewer for academic journals. His work aims to bridge scientific innovation and practical application to support sustainable aquaculture and improve the livelihoods of fish and shrimp farmers.

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Published

11.10.2025

Issue

Vol. 10 No. 2 (2025): Journal of Aquatropica Asia

Section

Articles

How to Cite

Renanda Baghaz Dzulhamdhani Surya Putra, R., Ayu Inggrida, J., Nicky Anugerah Putra, A., Setiawan, F., & Madha Su’a Achmadi, A. (2025). ANALISIS EXTRACELLULER PRODUCT Vibrio harveyi BERBASIS NANO-KITOSAN YANG DIPERKAYA EKSTRAK Caulerpa lentillifera SEBAGAI VAKSIN ORAL UNTUK KERAPU CANTANG TERHADAP INFEKSI Vibrio harveyi. Journal of Aquatropica Asia, 10(2), 139-147. https://doi.org/10.33019/pkq62x73