Efek toksisitas ekstrak bromelin buah nanas (Ananas comosus L.) dengan metode Brine Shrimp Lethality Test (BSLT)
Abstract
Bromelain is proteolytic enzyme extracted from pinepple (Ananas commosus L.) and potents as anticancer. The aimed of this study was to determined the toxicity effect of bromelain extract from pineapple fruit (Ananas commosus L) with brine shrimp lethality test (BSLT) methods. Crude extract of bromelain was obtained by addition of natrium acetat buffer pH 6.5 to pineapple fruit and was setrifugated with 3500 rpm of speed. Bromelain toxiciy was determined by incubated 15 artemia salina Leach (48 aged old) in various concentration of bromelain extract; 3.000 ppm, 2.000 ppm, 1.500 ppm, 1.000 ppm, 500 ppm and 0 ppm for 24 hours. At the end of incubation period, mortality percentages and LC50 were calculated. The result shown that there were increased in mortality percentage as bromelain concentration increased. LC50 value of bromelain exctract was 9.000 ppm. This value indicated that bromelain extract from pineapple fruit had low toxicity effect since its LC50 value were under 1000 ppm. Inconclusion, crude bromelain extract from pineapple fruit did not show anticancer potential based on this study.
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References
Astuti, P., Alam, G., Hartanti, M.S., Sari, D., Wahyuono, S. (2005). Uji sitotoksik senyawa alkaloid dadri Spons petrosia sp; potential pengembangan sebagai antikanker. Majalah Farmasi Indonesia, 16(1), 58-62
Bhattacharyya, B.K. (2008). Bromelain: an overview. Nat. Prod. Radiance, 7(4), 359-363
Chang, T.C., Wei, P.L., Makondi, P.T., Chen, W.T., Huang, C.Y., & Chang, Y.J. 2019. Bromelain inhibits the ability of colorectal cancer cell to proliferate via activation of ROS production and autophagy. PloS ONE, 14(1), e0210274.
Carballo, J.L., Inda, Z.L.H., Perez. (2002). A Comparison between two brine shrimp assay to detect in vitro cytotoxicity in marine natural product. BMC Biotechnol., 2(17), 1-5
Fajrin, E. (2012). Penggunaan Enzim Bromelin pada Pembuatan Minyak Kelapa (Cocos nucifera) secara Enzimatis. [skripsi]. Makassar: Universitas Hasanuddin.
Gautam, S.S., Mishra, S., Dash, V., Amit, K. and Rath, G. (2010). Cooperative study or extraction, purification and estimation of bromelain from stem and fruit of pineapple plant.Thai J. Pharm. Sci., 34 (1), 67-76.
Hairi, M. (2010). Pengaruh Umur Buah Nanas dan Konsentrasi Ekstrak Kasar Enzim Bromelin pada Pembuatan Virgin Coconut Oil dari Buah Kelapa Typical (Cocus nucifera L).[skripsi]. Malang; Jurusan Kimia Fakultas Sains dan Teknologi Universitas Islam Negeri Maulana Malik Ibrahim Malang.
Harmita, dan Radji, M. (2008). Analisis Hayati. Jakarta: Penerbit Buku Kedokteran.
Khuluq, M.N.N.H, Wardatun S, Wiendarlina, I.Y. (2017.) Uji toksisitas sari buah dan bonggol nanas (Ananas comosus (L.) Merr) terhadap larva udang (Artemia salina Leach). E-jurnal unpak. http://perpustakaan.fmipa.unpak.ac.id/file/ejurnal%20much%20066111161.pdf
Mayer, B.N., Ferigni, N.R., Putnam, J.E., Jacobsen, L.B., Nichols, D.E., McLaughin, J.L., (1982). Brine Shimp: A Convenient General Bioassay for Active Plant Constituent. Plant Medica, 45, 31-34
Shiew, P.S., Fang, Y.L. & Majid, F.A.A. 2010. In vitro study of bromelain activity inartificial stomach juice and blood. Paper presented at the 3rd Internasional Conference on Biotechnology for the Wellness Industry, PWTC Kuala Lumpur.
Mamo, J. & Assefa, F. 2019. Antibacterial and anticancer property of bromelain: a plant protease enzyme from pineapples (Ananas comosus). Curr. Trends Biomedical Eng & Biosci, 19(2), 0060-0068.
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